The Lashley III maze is a test used for learning and memory. It utilizes a low stress environment and looks into route learning of a maze under repeated trials. In typical protocols, no stress, food deprivation, or other stimuli are used. A start box, a maze body, and a goal box are contained in the MazeEngineers apparatus. The goal box can be filled with bedding similar to a home cage for reward for mice to motivate completion of the task. A large red base is not included in the order, but can be ordered upon request.


Price & Dimensions


$ 1890

Per Month
  • Arm Length: 45cm x 7cm height x 5cm width
  • Doors: 4cm x 4cm, located 11cm from walls
  • Goal box: 19.5cm long x 7cm height x 5cm width
  • Start box: 8cm x 9.5cm x 7cm
  • Clear rooftop.
  • Base does NOT come with the Lashley by default
  • Options: Red/Yellow/Blue/Black/Clear/White/Grey/Green


$ 1990

Per Month
  • Arm Length: 60cm x 10cm height x 8cm width
  • Doors: 6cm x 6cm, located 14cm from walls
  • Goal box: 26cm long x 9cm height x 6cm width
  • Start box: 10cm x 12cm x 9cm
  • Clear rooftop.
  • Base does NOT come with the Lashley by default
  • Options: Red/Yellow/Blue/Black/Clear/White/Grey/Green


Lashley III is a widely used alternation task to evaluate learning ability and memory in rodents. It relies on route learning in which subjects learn to explore a maze through repeated exposure. The apparatus was developed by Karl Lashley in 1929. He utilized the apparatus to discover memory engram within the cortex using the cortical lesioning techniques. This paradigm relies on low-stress conditions such as dark phase and no aversive stimulus until the subjects reach the goal box from the start box.

The apparatus consists of an integrated start box, a four-arm maze, and a goal box. In the modified versions, there is an addition of a pseudo-home cage at the end of the goal box. This pseudo-home cage consists of the same bedding as that of the subject’s original home cage and is kept specific for each subject during the entire course of the experiment. This supplemental home cage provides ample motivation to the subjects to learn the routes in Lashley III maze (Blizard et al. 2006)

Karl Lashley conducted the protocol relying on food cues for motivation, whereas later the food cued stimuli were changed with the pseudo home cage as it provides almost the same degree of motivation. Some investigators have also utilized a food pellet reward to encourage route learning in Lashley III maze (Matzel et al. 2003).

Apparatus and Equipment

The Lashley III maze is constructed of black acrylic covered by separate Plexiglas lids for every section to facilitate low light to simulate dark phase environment. The acrylic walls of the apparatus are non-transparent whereas the lids are transparent in nature. It consists of a start box, four maze arms with five decision points, eight blind alleys, and a goal box. However, the apparatus can also be augmented with a pseudo-home cage. The modular configuration of the apparatus not only assists in the easy cleaning of the apparatus, but it also assists to modify the apparatus as needed.

In general, the maze arms are approx. 45 cm long, 7 cm high, and 5 cm wide. The decision point doors are approx. 4 x 4 cm positioned approx. 11 cm from the outer walls of the apparatus. The start box and goal box are approx. 8 cm x 9.5 cm x 7 cm and 19.5 cm x 7 cm x 5 cm in dimensions respectively. The transparent Plexiglas lids not only allows the easy visualization of the subjects as they progress in the maze but also prevents the subjects to escape the maze. The maze usually lies on a red Plexiglas base that can hold the apparatus with the added pseudo-home cage approximately the same size as the subject’s standard home cage.

Training Protocol

Pre-training for Lashley III

Before the testing procedure, the subjects should be brought to the testing room for acclimation. For food cued motivation, the subjects are water and food deprived for some specific time usually 24-36 hours, but for the pseudo-home cage motivation, the water and food are generally removed half an hour before the testing. The procedure for Lashley III, especially when pseudo-home cage technique is employed for motivation is usually conducted during the dark phase as the subjects are food and water-satiated at this time. The dark phase provides an additional driving force to the subjects to explore the maze.

First, the maze should be thoroughly wiped with 70% ethanol solution. Initially, the doors of the start box leading to the maze arms should be kept closed. Then the subjects should be carefully placed in the start box, and the top lid is firmly secured to restrict the subjects from leaving the apparatus. Then the door leading to the maze arms should be opened, and stopwatch should be started. The pathway followed by the subject should be recorded either manually or through a video tracking software such as Noldus Ethovision XT. The latency to enter every arm, and the number of errors made should be properly documented.

For the manual recording of data, stop the stopwatch when all four paws of the subjects are in the first arm of the Lashley III maze. This time is recorded as the latency to enter the maze. When the subject reaches the goal box with its all four inside it, stop the second stopwatch, and the note down it as the latency to the enter the goal box. The doors that lead back to the maze arm should be immediately closed. Finally, stop the third stopwatch when the subject completely enters the pseudo home cage; record this time as the latency to enter the pseudo home cage.

Every time the subject enters the dead end or travels to the maze arm already traveled is taken as an error. These errors are referred as forward and backward errors respectively. The number of errors made by the subjects should be properly recorded. The manual recording of data is prone to human error; therefore the use of an automated video tracker such as Noldus Ethovision XT limits this problem efficiently.

The subject should be safely removed from the pseudo home cage after 1 minute before returning it to its standard home cage. The same process is repeated for all the subjects in the testing cohort. The subjects should be tested in the same order on consecutive trials.  All the subjects are tested on consecutive nights, with only one trail on each day for each subject. The testing procedure is repeated until the learning criterion is met. However, the testing procedure should be ceased after the predetermined test trails are completed, regardless the subjects achieve the learning criterion or not. The learning criterion is met when the subject navigates the maze and reaches the pseudo home cage making 0 or 1 error(s) on two successive trials.

Evaluation of the learning and retention domains

Lashley III maze can be used to evaluate the learning and retention domains. The learning ability is manifested by achieving the learning criterion. For the retention domain, the subjects are tested after seven days, and the latencies are recorded and compared to evaluate the retention ability of the subjects (Espinoza-Cifuentes and Zeise M, 2008).

Evaluation of aging in rodents

The effect of aging on the learning ability of the rodents can also be evaluated in the Lashley III maze. Bressler et al. 2010 slightly modified the apparatus to evaluate the factor of age in achieving the learning criterion.

Evaluation of effects of anteromedial/posterior parietal cortex lesions on spatial maze memory

The ibotenic acid lesions in blind rats limited only to the anteromedial complex region, but not to Oc1, interrupts spatial reference memory of the Lashley III maze (Pinto-Hamuy et al. 2004). They also observed a positive correlation between the extent of the lesion in the anteromedial complex and the intensity of the retention deficit.


The configurational modularity of the Lashley III permits the experimenter to test different characteristics of learning retention. Once the learning criterion is achieved, the Lashley III apparatus can be rotated to make the doors and choice points opposite to where they were before. Now the subject needs to learn the new route to establish learning criterion for this configuration.

The Lashley III maze can also be modified by adding odor cues or noise stimuli that serve as an additional driving force for motivation to encourage the exploration of the maze. In order to determine the state dependent learning and memory, aversive stimuli such as restraints can also be employed.

Fig1: Days to achieve learning criterion in 3 months and 20 month old subjects

The sample data can be visualized by comparing the time spent to achieve learning criterion in subjects with respect to age. It is evident from the graph that with the increasing age, the sensory capabilities are decreased; therefore, the older subjects spend more time to meet the learning criterion. Figure 1.

Fig 2: Relationship between AMC lesioned subject verses sham with respect to retention deficit.

Another sample data can be visualized by comparing the retention deficit domain of anteromedial lesioned subjects with sham group. Figure 2

Sample Data

Strengths & Limitations


The most exceptional advantage of the Lashley III maze is the modular nature of the apparatus. It can be simply rotated to make the choice points and doors opposite to where they were during the first acquisition phase.

Another advantage is its ability to evaluate the effects of aging on learning and memory. Although, Morris water maze is more efficient in determining learning and memory domains, lashley III evaluates the changes in the performance of the subjects more efficiently, especially when aging factor is under consideration. Other tests usually provide confounding results in aging rodents.


The duration of the training interval can prove to be detrimental for some subjects if they are over-trained. Over-trained subjects tend to show abnormal behavior in the maze which is manifested by the increased number of errors per trial, and prolonged durations to reach the learning criterion.

Summary and Key Points

  • Lashley III is a widely used alternation task to evaluate learning ability and memory in rodents.
  • Karl Lashley developed the apparatus in 1929.
  • The apparatus consists of a start box, four maze arms with five decision points, eight blind alleys, and a goal box.
  • The apparatus can be easily modified due to its modularity.


Bressler A, Blizard D, Andrews A. (2010). Low-stress route learning using the Lashley III maze in mice. J Vis Exp.  22;(39). pii: 1786. Doi: 10.3791/1786.

Lashley, K. (1929). Brain mechanisms and intelligence: A quantitative study of injuries to the brain. University of Chicago Press, Chicago.

Blizard DA, Weinheimer VK, Klein LC, Petrill SA, Cohen R, McClearn GE. (2006). ‘Return to home cage’ as a reward for maze learning in young and old genetically heterogeneous mice. Comp Med. 56(3):196-201.

Louis D. Matzel, Yu Ray Han, Henya Grossman, Meghana S. Karnik, Dave Patel, Nicholas Scott, Steven M. Specht and Chetan C. Gandhi. (2003). Individual Differences in the Expression of a “General” Learning Ability in Mice. Journal of Neuroscience. 23 (16) 6423-6433.

Espinoza-Cifuentes S, Leander Zeise M. (2008). The anteromedial extrastriate complex is critical for the use of allocentric visual cues and in the retention of the Lashley III maze task in rats. Biol Res. 41(4):405-12. Doi: /S0716-97602008000400006.

Pinto-Hamuy T, Montero VM, Torrealba F. (2004). Neurotoxic lesion of anteromedial/posterior parietal cortex disrupts spatial maze memory in blind rats. Behav Brain Res. 31;153(2):465-70.