Description

The empathy assay was first described in the literature by Jeon et al (2011) and is an excellent tool to interrogate observational fear. The MazeEngineers apparatus comes with two key components: A double-chambered acrylic chamber with two-foot shock-controlled grids, each of which can be controlled independently with our included Conductor Software or ConductVision tracking and analysis software. This apparatus is then placed inside of a standard, basic isolation chamber. Upgrades in this chamber to include sound and light cues are possible. Please inquire for more information.

The apparatus for the Empathy assay includes a sound-attenuating cubicle with a fan within which an observational fear-conditioning chamber is placed. The fear-conditioning chamber is composed of two chambers that are separated by a transparent Plexiglas divider. The chambers are 18 x 17.5 x 38 cm each with 5 mm diameter stainless steel rods (0.7 cm apart) placed parallel on the floor.

A  shocking system is also used to administer foot shock to the demonstrator conspecific. Two plates are included and both plates can be controlled separately.

Emotional empathy can be studied as a precursor to fear response arising from observing a conspecific experiencing an aversive stimulus. The novel environment tends to reduce the effect of the previous day’s fear conditioning during the 24-hour memory test.

Maze Engineers offer the Empathy Assay Test

The observational fear-conditioning assay is used to assess empathy in the subject by evaluating social observational fear learning. Empathy, in humans, is defined as the ability to recognize and share emotions with others. In many mental disorders such as Psychopathy and Antisocial Personality Disorder (ASPD), the individual’s empathetic abilities are impaired. Impairments in empathetic abilities of an individual can have a significant effect on their social-emotional health. Despite empathetic abilities being crucial to the quality of life of an individual, rodent models of emotional empathy impairments are rare, largely due to lack of robust behavioral assay systems.

Emotional empathy can be studied in rodents as a precursor to fear response arising from observing a conspecific experiencing an aversive stimulus. A simple protocol to observe social fear in rodents, suggested by Jeon et al., 2011, includes a two-chambered Fear Conditioning Chamber.  One of the chambers holds the observer subject, and the other holds a demonstrator conspecific that receives foot shocks. The chambers are separated by a clear wall to allow the subject to view the conspecific. The observer subject is observed for its response to the same set-up after 24 hours. Other assays that use subject sociability are the Sociability Chamber, the Social Defeat Apparatus and the Social Reward Chamber.

The apparatus for Empathy assay includes a sound-attenuating cubicle with fan within which an observational fear-conditioning chamber is placed. The fear-conditioning chamber is composed of two chambers that are separated by a transparent Plexiglas divider. The chambers are 18 x 17.5 x 38 cm each with 5 mm diameter stainless steel rods (0.7 cm apart) placed parallel on the floor.

A programmable, computer controlled shocking system is also used to administer foot shock to the demonstrator conspecific. Two plates are included and both plates can be controlled separately.

Before beginning the experiments, the equipment is cleaned to with 70% ethanol solution. Video recording and tracking software, such as Noldus Ethovision XT, are set-up, ensuring continual recording during the sessions.

Selection of Observer-Demonstrator pairs

• Non-sibling pair: Non-sibling subjects that are housed separately are used in the experiment of non-familiar animal observational fear conditioning.
• Sibling pair: Experimental pairs are chosen from the same group of siblings. The subjects have been maintained in groups of 2 or 3 per housing cage after weaning. As control demonstrators, an unrelated conspecific is used.
• Mating pair: One male and two female conspecifics are co-housed for 10 weeks or longer in mating cages. A pair consisting of the male and a female is chosen for the experiments.

The assay lasts for two days and is composed of two trials: Training trial and Memory test.

Training Trial

Place the subjects in their respective chambers facing the clear partition. The 5-minute habituation period is initiated, during which the demonstrator subject does not receive any foot shocks. Immediately after the habituation period begin the training trial. The demonstrator receives the foot shocks as programmed. The training trial lasts for 4-minutes, during which the observer subject will develop freezing behaviors as a response to the demonstrator subject showing typical behavioral responses (running, vocalization, and jumping) to the shocking.

24-hour Memory Test

At the end of the 9-minute training trial, house the observer-demonstrator pair individually for 24 hours. On the expiration of the 24 hours, place the observer subject back in the same chamber of the apparatus. As a memory response to previous day fear-conditioning, the observer subject will display freezing behaviors. Observe the subject for 4 minutes.

Evaluation of Observational Fear Learning and the Empathetic Response

Using C57BL/6J mice Jeon et al., 2011 performed experiments to assess empathy responses. Mice aged 10 to 15 weeks were used for experiments using sibling pairs and unfamiliar pairs. Another set of a mating pair was also used in the experiments. The familiar pairs were separated just before the experiments. In their experiments, they observed that observational fear-conditioning elicited the same freezing behaviors under both the training and 24-hour contextual memory trials. Further, it was observed that familiar pairs (sibling and mating) showed a higher degree of freezing behaviors as compared to the unfamiliar pairs.

The following data are observed during the Empathy Assay.

• Duration of freezing behaviors during training trial
• Duration of freezing behavior during 24-hour memory trial
• Number of fecal droppings (an indirect measure of fear response)

Freezing behavior is characterized by the lack of movement, except for respiratory movements for 2 seconds or longer. Familiar pairs tend to show heightened freezing responses.

Strengths

The Empathy assay brings us one step closer to understanding how human empathy works. The test analyzes empathy as a precursor to observational fear conditioning. It can be observed that subjects that are familiar with the demonstrator conspecific show heightened response to their sufferings. It can also be seen that co-housing period for the pairs has a graded effect on the development of observational fear, with longer periods resulting in stronger responses. The test also allows analyzing the effect of novel environment (such as placing the observer in the other compartment or using an opaque divider wall) in the 24-hour contextual memory test. It can be seen that changing the environment for the memory test shows a reduced memory-based fear response to the previous day fear-conditioning. The test can be easily adapted to study different scenarios of empathy-based behaviors, such as observing the performance of observers who have experienced aversive stimulus before the Empathy assay (Atsak et al., 2011).

Limitations

For the trials, it is important not to use juveniles or young adults as there are likely to show reduced responses. Further, pairings (exception of mating pairs) should be of same-sex conspecifics with similar size and weight. It is important to house the observer and the demonstrator separately after the habituation and training period to prevent the social transfer of fear. It must be understood that this model cannot take into account the large range of human emotions; thus care must be taken while interpreting the data for translational research. Other factors that can influence the performance of the subjects may include the co-housing periods for sibling and mating pairs, handling procedures and presences of any undesired cues (olfactory, auditory or visual).

• Emotional empathy can be studied as a precursor to fear response arising from observing a conspecific experiencing an aversive stimulus.
• Freezing behavior is characterized by lack of movement, except for respiratory movements for 2 seconds or longer.
• Familiarity between the observer and the demonstrator leads to a heightened fear response from the observer when the demonstrator is subjected to aversive stimulus.
• Co-housing period of sibling and mating pairs has a significant effect on the fear responses.
• Subjects of the same size and weight should be paired to avoid any abnormal behaviors.
• The novel environment tends to reduce the effect of previous day fear conditioning during the 24-hour memory test.
• Pairs should not be housed together after the habituation and training trial to avoid social transfer of fear.
• Care should be taken to avoid influencing the observers during the 24-hour waiting period before the memory test.
• It must be understood that animal models cannot fully portray the entire range of human emotions.