Open Field
Open Field
Open Field
open field test

Description

The Automated Open Field is an automated system used to observe, record, and analyze rodents’ locomotor and behavioral activity levels. The system consists of an open field box, surrounded by an outer area divided into quadrants with activity integration that reports the animal location using photo-array technology. It is an adapted version of the Open Field arena which includes sensors for recording and software for analyzing rodents’ behavior.  It can measure the subject’s locomotion, behavioral activity levels, for behaviors associated with anxiety, exploration, and thigmotaxis.

It can be used to assess the effect of age on locomotion, neuromuscular abnormalities of genetically modified animals, the effect of therapeutic interventions on locomotion and behavior, etc.

The system is similar to the Activity Hole Board and General Activity Systems.

Mouse

$ 3295

Per Month
  • 40 x 40 x 30 cm (L x W x H)
  • Photoarray sensors:
  • (X, Y) move sensor frame (labelled X, Y)
  • Vertical jump senor frame (labelled Z)

Rat

$ 3795

Per Month
  • 60 x 60 x 40 cm (L x W x H)
  • Photoarray sensors:
  • (X, Y) move sensor frame (labelled X, Y)
  • Vertical jump senor frame (labelled Z)

Activity Measurements and Data Analysis

Animal's location

Real-time location measurements are recorded throughout the given experiment time. Adjustable sensors report location data using X and Y coordinates. X and Y coordinates can be scaled to the Activity system's physical dimensions (40 x 40 cm for mice and 60 x 60 cm for rats) for real-time location measurements.

Path creation

Tracked paths in real-time and throughout the experiment. A visualization of the path made by the animal subject is observed within the software under activity data using X and Y coordinates (see the image below ).

Locomotion activity

Users can choose a time interval post experiment and draw an activity chart indicating the location, number, and timing of jumps as time, and count points. For example, (10,4) indicates 4 jumps within 10 seconds. The following results are recorded:
When a jump occurs
When the animal moves without a jump
When the animal moves while in the jump position
Number of times the animal jumped during a given duration

Activity data visualized using Automated Open Field software including animal path and past/current locations.

Documentation

Introduction

The open-field arena is a widely used apparatus for assessing rodents’ locomotion, exploratory behavior, and anxiety. Our Automated Open Field can be used to observe, record, and analyze rodents’ locomotor and behavioral activity levels. The system is an adapted version of the Open Field arena and includes software for analyzing rodents’ locomotion and behavior and can record when an animal moves with a jump and without a jump. Moreover, the experimenter can set a time interval for recording the number of jumps in a given time period, which is illustrated in a “Jump Chart”. The software also provides a visual illustration of the animal’s locomotion during each trial. 

This activity system can effectively be used as an in-vivo locomotion assay for various experiments. It can be used to assess the effect of age on locomotion behavior, neuromuscular abnormality of genetically modified animals, anxiety-related behaviors on activity levels and the effect of therapeutic interventions on locomotion and behavior. 

Apparatus and Equipment

The Activity System comprises an open field arena. It is available with separate measurements for rats and mice. It measures 60 × 60 × 40 cm (L × W × H) for rats and 40 × 40 × 30 cm for mice. The floor of the arena contains 16 holes spaced evenly apart. The diameter for each hole is 3 cm for rats and 2 cm for mice. The sensor’s height within each hole is adjustable. The walls of the arena are made of plexiglass. 

It also includes software to measure and record the animal’s location and make a visual drawing of the activity data. 

Installation

Software

Installation of the software is simple and easy with standard download and installation steps.

Hardware

The Activity box can easily be connected to your computer using a standard USB cable. Connection occurs once your PC has recognised the apparatus.

Experiment configuration

The user interface is easy to use with configurable parameters.

Experiment run

Results summaries report the animal's location, visualise their path throughout the experiment, jumps, time intervals, nose pokes, and more!

Support

Maze Engineers support and troubleshooting is provided throughout your experiment and set-up.

Training Protocol

  1. Place the subjects in the testing room in their home cages for 10 – 30 minutes to acclimate. 
  2. Configure the software and set the primary data collection parameters.
  3. Remove the subject from its home cage and place it in the activity system once the software is configured. 
  4. Select the appropriate command in the software and begin data collection.
  5. Leave the testing room for the rest of the testing duration.
  6. After the testing period is completed, return to the room and save the data. 
  7. Return the subject to its home cage. 

Literature Review

Analysis of Locomotor Activity in Rodents 

Zhang, Li, and Han (2020) analyzed the locomotor activity of mice using an activity system. The system comprised an open field, a webcam, and a computer with software. The distance traveled, velocity, and thigmotaxis of the animals were readily obtained using the apparatus. 

Aragão et al. (2011) analyzed locomotor activity in rodents using an automated activity system. The system consisted of an open field with 30 cm high walls. It utilized digital images captured under infrared red of freely moving rodents to measure their activity. Moreover, videos of the rodents were analyzed using the software. Fifteen male Wistar rats were used in the study. The distance traveled, average speed, average potency, time spent immobile, number of stops, and length of stay in the area of the subjects within the apparatus were recorded. The animals were evaluated on multiple postnatal days to investigate the study’s reproducibility. The results indicated that all parameters were effectively measured using the system. Moreover, development changes in the patterns of movement in the subjects were also observed across postnatal days. 

Summary

  • The Activity System is an automated system used to observe, record, and analyze rodents’ locomotor and behavioral activity levels.
  • It is an adapted version of the Open Field arena which includes sensors for recording and software for analyzing rodents’ behavior. 
  • It can measure the subject’s locomotion, behavioral activity levels, anxiety, exploration, and thigmotaxis.
  • The system can be used to assess the effect of age on locomotion, neuromuscular abnormalities of genetically modified animals, the effect of therapeutic interventions on locomotion and behavior etc. 

References

Aragão, R., Rodrigues, M. A., de Barros, K. M., Silva, S. R., Toscano, A. E., de Souza, R. E., & Manhães-de-Castro, R. (2011). Automatic system for analysis of locomotor activity in rodents–a reproducibility study. Journal of neuroscience methods, 195(2), 216–221. https://doi.org/10.1016/j.jneumeth.2010.12.016

Zhang, C., Li, H., & Han, R. (2020). An open-source video tracking system for mouse locomotor activity analysis. BMC research notes, 13(1), 48. https://doi.org/10.1186/s13104-020-4916-6